NAD-dependent recombinant ligase from Thermus thermophilus. The ligation will occur only if oligonucleotides are perfectly paired to the complementary target DNA and have no gaps between them. Therefore, a single-base substitution can be detected. High thermostability allows ligation using high-stringency hybridization conditions. High specificity and stringency permits sensitive detection of SNPs. Equivalent of Ampligase® (Epicentre).
Manufacturer: BLIRT S.A.