IDEAL II DNA Ladder ready-to-use

Index Size
MR25 100 lanes

Product Features

Molecular weight standard specially designed for electrophoretic analysis of medium and large DNA fragments on agarose and non-denaturing polyacrylamide gels. Ideal for sizing of PCR products, digested plasmids and medium or large double-stranded DNA fragments. IDEAL DNA ladder contain purified blunt-ended PCR products and cohesive-ended restriction fragments. The ladder is premixed with 6x Green DNA Loading Dye for direct loading onto agarose and non-denaturing polyacrylamide gels.The optimized 6x Green DNA Loading Dye contains two electrophoresis tracking dyes: orange G and xylene cyanol FF. Orange G migrates at approximately 50 bp on a standard 1% TAE or TBE agarose gels, whereas xylene cyanol FF migrates at approx. 4 kbp.

 

Contents

- IDEAL ready-to-use DNA Ladder (250 µl)

- 6x Green DNA Loading Dye (500 µl)

 

Features

- ideal, because in wide range clear

- ready-to-use - premixed with 6x Green DNA Loading Dye for direct loading onto agarose and non-denaturing polyacrylamide gels 

- allow precise sizing of medium and large DNA fragments

- produce sharp bands during gel electrophoresis

- can be visualized under UV light after ethidium bromide, SYBR Green or SYBR Gold staining

- loading dye solution is included

 

DNA fragment lengths (bp):

9276, 5982, 3906, 2688, 2000, 1500, 1000, 700

 

Storage Buffer
10 mM Tris-HCl (pH 7.6), 0.005% xylene cyanol FF, 0.025% orange G, 2.5% Ficoll® 400 and 10 mM EDTA

 

6x Green DNA Loading Dye (supplied with the ladder)
20 mM Tris-HCl (pH 7.6), 0.03% xylene cyanol FF, 0.15% orange G, 15% Ficoll® 400 and 60 mM EDTA

 

Loading

- we recommend loading 5 µl of ready-to-use DNA ladder per gel lane

- do not heat before loading

- mix 1 volume of the dye with 5 volumes of the DNA sample

 

Quality Control
- tested in appropriate gel electrophoresis

- the absence of nucleases is confirmed by appropriate tests

 

Storage
Store at room temperature or at 4°C. For longer periods, store at -20°C.

 


Manufacturer: BLIRT S.A.