IDEAL II DNA Ladder

Index Size
MR22 100 lanes

Product Features

Molecular weight standard specially designed for electrophoretic analysis of medium and large DNA fragments on agarose and non-denaturing polyacrylamide gels. Ideal for sizing of PCR products, digested plasmids and medium or large double-stranded DNA fragments. IDEAL DNA ladder contain purified blunt-ended PCR products and cohesive-ended restriction fragments.

 

DNA Ladders are supplied with 6x Green DNA Loading Dye (500 µl).

 

Features

- ideal, because in wide range clear

- allow precise sizing of medium and large DNA fragments

- produce sharp bands during gel electrophoresis

- can be visualized under UV light after ethidium bromide, SYBR Green or SYBR Gold staining

- loading dye solution is included

 

DNA fragment lengths (bp):

9276, 5982, 3906, 2688, 2000, 1500, 1000, 700

 

Storage Buffer (TE buffer)
10 mM Tris-HCl (pH 7.6) with 1 mM EDTA

 

6x Green DNA Loading Dye (supplied with the ladder)
20 mM Tris-HCl (pH 7.6), 0.03% xylene cyanol FF, 0.15% orange G, 15% Ficoll® 400 and 60 mM EDTA

 

Loading

- mix 1 volume of the dye with 5 volumes of the DNA solution

- do not heat before loading

- we recommend loading 5 µl of DNA ladder per gel lane

 

Quality Control
- tested in appropriate gel electrophoresis

- the absence of nucleases is confirmed by appropriate tests

 

Storage
Store at room temperature or at 4°C. For longer periods, store at -20°C (recommended).

 


Manufacturer: BLIRT S.A.