Thermostable DNA polymerase Hypernova
Hypernova DNA Polymerase is a unique blend of a modified highly thermostable and proofreading DNA polymerase Pwo isolated from the hyperthermophilic archaeon Pyrococcus woesei and enzymes increasing yield and performance of the PCR reaction. The enzyme mix can generate very long amplicons (over 10 kb). Such long amplicons are often difficult to generate with a single DNA polymerase. Hypernova is a versatile and easy-to-use polymerase, since it works with many different protocols and requires minimal time consuming optimization. The polymerase produces higher yields than most commercially available enzymes and is ideally suited for difficult PCR templates. For its exceptional stability recommended also for diagnostic PCR in the case when long reactions and high sensitivity are required. The polymerase maintains 95% activity even after 40 cycles consisting of three one minute steps each.
- Extreme processivity (for very long amplicons)
- Extreme yield with minimal amounts of enzyme and little optimization
- Extreme fidelity (proofreading activity)
- Ideal for problematic templates that fail with standard Taq DNA polymerases
- Foolproof during multiplex PCR
- Very specific and sensitive
- More thermostable than Taq polymerase
- Very low price!
- Long range PCR
- Production of higher yields of PCR products
- High fidelity for purposes of cloning, expression, site-directed mutagenesis
- Multiplex PCR
- PCR from GC-rich or difficult templates
- Blunt-end PCR cloning
- Diagnostic PCR
Hypernova DNA Polymerase is supplied with 10x Hypernova and 10x Shark Buffer and 50 mM MgCl2 solution.
10x Hypernova Buffer (without Mg2+)
100 mM Tris-HCl (pH 8.3 at 25°C), 500 mM KCl, 1.5% Triton X-100
10x Shark Buffer (without Mg2+)
200 mM Tris-HCl (pH 8,3), 100 mM KCl, 100 mM (NH4)2SO4, 1,0% Triton X-100
Suggested PCR reaction mix (50 μl):
10 mM Tris-HCl (pH 8.3), 50 mM KCl, 0.15% Triton X-100, 200 μM each dNTP, 3.4 mM MgCl2, 0.1 μg DNA, 0.4 μM each primers and 0.4-1.0 units of Hypernova DNA Polymerase.
20 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol
Shipping and Storage conditions:
Routine storage at -20ºC. Shipping and temporary storage at room temperature has no detrimental effects on the quality of Hypernova DNA Polymerase.
Free of detectable nonspecific nucleases and DNA contamination. Extensively tested for PCR applications.
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 75°C in a total volume of 50 μl.
Manufacturer: BLIRT S.A.