EXTRACTME RNA BACTERIA & YEAST KIT

Index Size
EM25-010 10 preps
EM25-050 50 preps
EM25-250 250 preps

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Product Features

The EXTRACTME RNA BACTERIA & YEAST kit is designed for the rapid and efficient purification of high quality total RNA from broth yeast or bacteria cultures as well as frozen cells. The isolation protocol and buffer formulations were optimized for high isolation efficiency and purity of RNA. The product is intended for research use only.

 

SAMPLE MATERIAL

- broth bacterial (up to 1x 109 cells) or yeast (up to 5x 107 cells) culture
- frozen cell pellet

 

YIELD

It depends on the sample material amount and type.
- up to 60 μg RNA from bacterial culture
- up to 30 μg RNA from yeast culture

 

BINDING CAPACITY

approx. 90 μg RNA

 

TIME REQUIRED

- 30 minutes for RNA isolation from bacteria
- 50 minutes for RNA isolation from yeast

 

RNA PURITY

A260/A280 ratio = 1.9 – 2.1

 

Principle

The EXTRACTME RNA BACTERIA & YEAST kit utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. In the first step of the isolation procedure the cell walls are disintegrated and the membranes and proteins are degraded. Any RNases are inactivated by guanidine thiocyanate and β-mercaptoethanol. The homogenate is separated from the undigested cell remains by centrifugation and on the Homogenizing Column. The RNA is bound to the Purification Column membrane by addition of ethanol. The three-step washing stage effectively removes impurities and enzyme inhibitors. The purified RNA is eluted using a low ionic strength buffer or RNase-free water (pH 7.0-9.0) and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth.

 

Quality control

The quality of each production batch (LOT) of the EXTRACTME RNA BACTERIA & YEAST kit is tested using standard QC procedures. The purified RNA concentration, quality and stability are evaluated by gel electrophoresis and spectrophotometer. In addition, the functional quality is tested by reverse transcription and qPCR.


Manufacturer: BLIRT S.A.