|EM21-192||2 plates (2x 96 preps)|
|EM21-960||10 plates (10x 96 preps)|
The EXTRACTMEPLASMID DNA 96-WELL KIT is designed for high-throughput and
efficient purification of high quality plasmid DNA from recombinant Escherichia
coli strains. The isolation protocol and buffer formulations were optimized for
high isolation efficiency and DNA purity. Additionally, this protocol consists of
centrifugation or vacuum based procedure that omits conventional cell pelleting
and resuspention steps. The product is intended for research use only.
up to 1.5 ml bacterial culture per well
5-20 µg pDNA per well
Approx. 20 μg DNA per well
A260/A280 ratio = 1.7 – 1.9
standard SBS footprint
- Centrifuge with rotor for plates (≥3k x g);
- Automatic Liquid Handling Systems (eg. Biomek® FX, TECAN Freedom Evo, TECAN Genesis, Eppendorf epMotion)
The DNA purification procedure utilizes spin 96-minicolumns plates with
membranes which efficiently and selectively bind nucleic acids. In the first isolation
step, the plasmid DNA is released from bacterial cells by alkaline lysis. Then the
lysate is neutralized and all the cell residues along with the proteins and genomic
DNA are separated. The lysate is applied to the purification minicolumn membrane
and the DNA is bound. The two-step washing stage effectively removes impurities
and enzyme inhibitors. The purified plasmid DNA is eluted using a low ionic
strength buffer (Elution Buffer) or water (pH 7.0-9.0) and may be used directly in all
downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing,
enzymatic restriction, ligation and so forth, or stored until ready to use.
The quality of each production batch (LOT) of the EXTRACTME PLASMID DNA 96-WELL kit is tested using BLIRT’s ISO-certified quality management system. The purified DNA concentration, quality and stability are evaluated by gel electrophoresis and spectrophotometry. In addition, the functional quality is tested by qPCR, digestion with restriction enzymes and pDNA transfection.
Manufacturer: BLIRT S.A.