EXTRACTME DNA SWAB & SEMEN KIT

Index Size
EM06-010 10 preps
EM06-050 50 preps
EM06-250 250 preps

Download protocol

Product Features

The EXTRACTME DNA SWAB & SEMEN kit is designed for the rapid and efficient purification of high quality genomic DNA from human and animal mucosa membrane swabs (including buccal, nasal, pharyngeal and vaginal swabs), saliwa as well as from semen. The isolation protocol and buffer formulations were optimized for high isolation efficiency and DNA purity.

 

SAMPLE MATERIAL

buccal, nasal, pharyngeal, vaginal, blood and saliva swabs or semen

 

EFFICIENCY

Dependent on the sample material amount and type. Up to 3 μg DNA from a swab and up to 7 μg from 150 μl of a semen sample.

 

BINDING CAPACITY

approx. 25 μg DNA

 

TIME REQUIRED

40-50 minutes (including incubation time)    

 

DNA PURITY

A260/A280 ratio = 1.7 – 1.9

 

Principle

The DNA purification procedure consists of four steps and utilises spin minicolumns with membranes which efficiently and selectively bind nucleic acids. The swab or semen sample is subjected to enzymatic lysis by Proteinase K in the SSL buffer. When isolating from semen, DTT must also be used. In this step, the cell walls, membranes and proteins are degraded by lysis buffer and Proteinase K. After the addition of chaotropic salts, the lysate is applied to the purification column membrane and the DNA is bound. The two-step washing stage effectively removes impurities and enzyme inhibitors. The purified DNA is eluted using either a low ionic strength buffer or water (pH 7.0-9.0) and can be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use.

 

Quality control

The quality of each production batch (LOT) of EXTRACTME DNA SWAB & SEMEN kit is tested using standard QC procedures. The purified DNA concentration, quality and stability are evaluated by gel electrophoresis and spectrophotometer. In addition, the functional quality is tested by qPCR and digestion with restriction enzymes.


Manufacturer: BLIRT S.A.