The EXTRACTME DNA CLEAN-UP & GEL-OUT kit is designed for the rapid and efficient purification of DNA fragments after enzymatic reactions (removal of nucleases, enzyme inhibitors, detergents, restriction enzymes, polymerases, divalent ions, salts etc.) and for the purification of DNA fragments directly from agarose gels (standard and low-melting point agarose gels run in either a TAE or TBE buffer). The kit enables the purification of DNA fragments from 50 bp to 30 kb, as well as plasmid and genomic DNA. However, purification of fragments smaller than 100 bp and larger than 10 kb will result in decreased recovery rates. The purified DNA can be used in common downstream applications. The purification protocol and buffer formulations were optimized for high yields and DNA purity.
CLEAN-UP: up to 100 μl of a DNA sample
GEL-OUT: up to 300 mg of agarose fragment containing DNA
CLEAN-UP: 90-99%, depending on DNA fragment length (in the range of 100 bp – 10 kb)
GEL-OUT: 70-95%, depending on DNA fragment length (in the range of 100 bp – 10 kb)
DNA FRAGMENT LENGTH
- 100 bp - 10 kb
- DNA fragments in the 50-100 bp and 10-30 kb range can also be purified, as can genomic and plasmid DNA. However, the efficiency will be decreased.
approx. 25 μg DNA
CLEAN-UP: 5-10 minutes
GEL-OUT: 16-20 minutes
The quality of each production batch (LOT) of EXTRACTME DNA CLEAN-UP & GEL-OUT kit is tested using standard QC procedures. The purified DNA concentration, quality and stability are evaluated by gel electrophoresis and spectrophotometer. In addition, the functional quality is tested by PCR and qPCR.
Manufacturer: BLIRT S.A.