EasyPCR HIV

Index Size
DY255A 5 exercises
DY25A 1 exercise

Product Features

EasyPCR HIV - educational kit for determination of HIV resistance by PCR reaction.  

 

AIM: The purpose of the exercise is to carry out a PCR reaction in order to detect the mutation in the ccr5 gene which confers resistance to HIV in humans. The detection is based on an amplification of ccr5 gene fragments:

- 220 bp in homozygotes without the mutation

- 188 bp in homozygotes with the mutation

- 220 and 188 bp in heterozygote with the mutation

 

The kit is designed to perform experiments during two laboratory classes (I - PCR set-up - 1.5 hours; II - electrophoresis - 1.5-2 hours). We suggest to perform laboratory experiments in six groups of two students.

 

EasyPCR HIV kit components (1 exercise, DY25A):

6 sets of reagents for PCR:  

- 6 tubes with 10x reaction buffer (10 reactions per tube)

- 6 tubes with MgCl2 solution (10 reactions per tube)

- 6 tubes with ready-to-use dNTPs mixture (10 reactions per tube)

- 6 tubes with FORWARD primer (10 reactions per tube)

- 6 tubes with REVERSE primer (10 reactions per tube)

- 6 tubes with PCR - grade water

- 6 tubes with thermostable DNA polymerase (10 reactions per tube)

- 6 tubes with Positive control gene: 220 bp fragment (5 reactions per tube)

- 6 tubes with Positive control gene: 188 bp fragment (5 reactions per tube)

- 12 tubes with DNA samples for analysis (3 reactions per tube)

 Moreover:

- 7 laboratory protocols (one with additional information for a teacher)

- Agarose (5 g)

- Buffer for agarose electrophoresis - 50x TAE (50 ml)

- Ready-to-use M100-500 DNA Ladder for 10 electrophoretic lanes

- DNA Gel Loading Buffer - 6x GREEN (0.5 ml)

 

EasyPCR HIV kit components (5 exercises, DY255A):

6 sets of reagents for PCR:  

- 6 tubes with 10x reaction buffer (50 reactions per tube)

- 6 tubes with MgCl2 solution (50 reactions per tube)

- 6 tubes with ready-to-use dNTPs mixture (50 reactions per tube)

- 6 tubes with FORWARD primer (50 reactions per tube)

- 6 tubes with REVERSE primer (50 reactions per tube)

- 6 tubes with PCR - grade water

- 6 tubes with thermostable DNA polymerase (50 reactions per tube)

- 6 tubes with Positive control gene: 220 bp fragment (25 reactions per tube)

- 6 tubes with Positive control gene: 188 bp fragment (25 reactions per tube)

- 12 tubes with DNA samples for analysis (15 reactions per tube)

 Moreover:

- 7 laboratory protocols (one with additional information for a teacher)

- Agarose (25 g)

- Buffer for agarose electrophoresis - 50x TAE (100 ml)

- Ready-to-use M100-500 DNA Ladder for 50 electrophoretic lanes

- DNA Gel Loading Buffer - 6x GREEN (1 ml)

 

Additional equipment and materials required (not included in the kit):

- Automatic pipettes with appropriate tips

- PCR and 1.5 ml Eppendorf tubes

- UV transilluminator or lamp

- Thermal cycler

- Microwave oven or laboratory burner

- Agarose electrophoresis equipment (apparatus, power supply)

- Ethidium bromide solution or other stain for DNA visualization

- Nitrile gloves

 

Shipping condition

Shipping or dry or blue ice.

 


Manufacturer: BLIRT S.A.