6x Orange DNA Gel Loading Dye is pre-mixed loading buffer with a tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis. This solution contains EDTA that binds divalent metal ions, inhibits metal dependent enzymatic reactions and protects DNA from metal dependent nucleases, effectively inactivating enzymes and protecting DNA from nucleases. The loading dyes increase the density of the sample and they add colour to the sample, thereby simplifying the loading process. Orange G will not appear in gel photographs, and runs ahead of all but the smallest restriction fragments. Orange G migrates at approximately 50 bp on a standard 1% TAE or TBE agarose gels.
6x Orange DNA Gel Loading Dye
20 mM Tris-HCl (pH 7.6), 0.15% orange G, 15% Ficoll® 400 and 60 mM EDTA
Use 1 µl of 6x Orange Gel Loading Dye per 5 µl of DNA sample. Mix well before loading onto a gel.
Tested for DNA sample preparation prior to agarose gel electrophoresis.
Store at room temperature or at +4°C up to 12 months. For longer periods, store at -20°C.
Manufacturer: BLIRT S.A.