phi29 DNA Polymerase (EN23)

Very processive polymerase (up to 70 kb) with strong strand displacement activity, which allows for highly efficient isothermal DNA amplification; possesses a 3’ 5’ exonuclease (proofreading) activity acting preferentially on ssDNA or RNA, therefore 3’-modified primers are recommended.

more ››

EN23-010 1000 U

EN23-050 5000 U

RIBOPROTECT Hu RNase Inhibitor (RT35)

Recombinant human placental RNase inhibitor expressed in E. coli strain.

more ››

RT35-020 2000 U

RT35-100 10000 U

RIBOPROTECT Mu RNase Inhibitor (RT37)

Recombinant protein of murine origin, that is more resistant to oxidation compared to the human or porcine version and is stable at low DTT concentrations.

more ››

RT37-020 2000 U

RT37-100 10000 U

Tth DNA Ligase (EN13)

NAD-dependent recombinant ligase from Thermus thermophilus. The ligation will occur only if oligonucleotides are perfectly paired to the complementary target DNA and have no gaps between them. Therefore, a single-base substitution can be detected. High thermostability allows ligation using high-stringency hybridization conditions. High specificity and stringency permits sensitive detection of SNPs. Equivalent of Ampligase® (Epicentre).

more ››

EN13-025 250 U

EN13-250 2500 U

UDGase (EN19)

Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides. Widely used to control carry-over contamination in PCR. Concentration 1 U/μl.

more ››

EN19-050 500 U

EN19-250 2500 U

TRANSCRIPTME M-MuLV Reverse Transcriptase (RT32)

Modified M-MuLV Reverse Transcriptase; 10 pg –5 μg of total RNA; concentration 200 U/μl; has increased thermal stability (optimum activity at 50°C); has no 3’-5’ exonuclease or RNase H activity, which improves the synthesis of a fulllength cDNA, even from long mRNA templates, using random priming; gives high yields of first strand cDNA up to 10 kb long.

more ››

RT32-010 10000 U

RT32-050 50000 U

T4 DNA Ligase (EN11)

ATP-dependent recombinant enzyme used for molecular cloning, site-directed mutagenesis, nick repair in duplex DNA, RNA or DNA/RNA hybrids, Ligation Mediated PCR; concentration 5 U/μl.

more ››

EN11-050 500 U
(5 U/µl)

EN11-250 2500 U
(5 U/µl)

Quick Ligase (EN12)

ATP-dependent recombinant T4 DNA ligase for efficient ligation of DNA fragments with compatible cohesive or blunt ends in 5 and 15 minutes respectively.

more ››

EN12-050 50 reaction

EN12-150 150 reaction

Proteinase K (RP101)

Mutagen and recombinant Proteinase K from Tritirachium album is non-specific, subtilisin-related serine protease with a very high specific activity.

more ››

RP100B 100 mg

RP101B 250 mg

RP102B 1000 mg

RP105B 1 ml (10 mg/ml)

RP107B 1 ml (20 mg/ml)

TEV Protease (RP171)

Recombinant cysteine protease from Tobacco Etch Virus mainly used f or removal of affinity markers from fusion proteins; enzyme concentration: 5 U μl.

more ››

RP171 1000 U

RP172 10000 U

RNase H (RT34-025)

Recombinant enzyme, which hydrolyses specifically the phosphodiester bonds of RNA hybridized to DNA; it is a key enzyme in the removal of mRNA after first-strand cDNA synthesis.

more ››

RT34-025 250 U
(5 U/µl)

RT34-125 1250 U
(5 U/µl)

BSA (Bovine Serum Albumin) (EN17)

A highly pure Albumin (Fraction V) recommended for a variety of applications where quality is required; purity >98%; Heavy Metals
(Pb) <0.001%; free of nucleases and proteases; soluble in water; pH (5% in water at 25°C) 6.5-7.5.

more ››

EN17-010 10 g

EN17-100 100 g